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The antibody is from purified rabbit serum by affinity purification through sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of the gene has actually been observed in many tumors, which leads to chromosome instability, and so may possibly contribute to tumorigenesis. This protein was found to affiliate with, and be involved in, the phosphorylation of NPAT protein (nuclear protein mapped into the ATM locus), which participates in mobile-cycle regulated histone gene expression and performs a essential function in promoting cell-cycle development during the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinct isoforms, are described. Two supplemental splice variants have been reported but detailed nucleotide sequence information and facts will not be nevertheless obtainable.
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Blocking peptides are peptides that bind precisely to the focus on antibody and block antibody binding. These peptide commonly has the epitope recognized through the antibody.
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This tutorial outlines even more achievable optimization of cellular and immunoassay parameters to be certain the absolute best final results are...
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This tutorial outlines more doable optimization of mobile and immunoassay parameters to guarantee the very best success are...
Antibodies bound to the blocking peptide no more bind into the epitope over the focus on protein. By evaluating the staining from your blocked antibody as opposed to the antibody by yourself, one can see which staining is certain.
Goal Details Cyclin E belongs towards the really conserved cyclin spouse and children, whose customers are characterised by a remarkable periodicity in protein abundance with the mobile cycle. Cyclins purpose as regulators of CDK kinases. Distinctive cyclins show distinct expression and degradation styles which lead for the temporal coordination of each mitotic party. Cyclin E varieties a complex with and functions as being a regulatory subunit of CDK2, whose exercise is required for cell cycle G1/S transition. This protein accumulates within the G1-S period boundary and it is degraded as cells development by way of S stage.
The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by both equally antibodies...
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